I have an Acolyte encounter card in play with 1 doom on it. Counting the doom on that threshold, I've now hit the Doom Threshold, which will advance the Agenda.

Question: When I remove the 4 doom it took to reach the threshold, do I also remove the 1 doom on the Acolyte still in play?

I would think so, but would like to confirm.

Yes, doom is removed from all cards in play when in threshold is reached unless otherwise specified.

Thanks man! Much appreciated.